0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
RPTOR
Application Dilute:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Raptor) Polyclonal Antibody, Unconjugated (orb6836) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Raptor) Polyclonal Antibody, Unconjugated (orb6836) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Raptor) Polyclonal Antibody, Unconjugated (orb6836) at 1:200 overnight at 4C, followed by a conjugated secondary (orb868589) at 1:500 for 90 minutes and DAPI staining of the nuclei.
Sample: A549 (human) cell Lysate at 40 ug, Primary: Anti-Raptor (orb6836) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 147kD, Observed band size: 135 kD.
Sample: Placenta (mouse) Lysate at 40 ug, Primary: Anti-Raptor (orb6836) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 147kD, Observed band size: 135 kD.
Immunohistochemical staining of rat small intestine tissue using Raptor antibody.
Immunohistochemical staining of mouse placenta tissue using Raptor antibody.
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