RIPK3 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6881
Article Name: RIPK3 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6881
Supplier Catalog Number: orb6881
Alternative Catalog Number: BYT-ORB6881-100,BYT-ORB6881-200,BYT-ORB6881-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human RIPK3 (101-230/518aa)
Conjugation: Unconjugated
Alternative Names: RIP3, RIPK3_HUMAN, RIPK3, RIP-like protein kinase 3, Receptor-interacting protein 3 (RIP-3), 2.7.11.1, RIPK3_MOUSE, Receptor-interacting protein 3 (RIP-3 | mRIP3),
RIPK3 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 57 kDa
UniProt: Q9Y572
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: RIPK3
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RIPK3) Polyclonal Antibody, Unconjugated (orb6881) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Sample: A549 cells lysates, 30 ug, Primary: Anti-RIPK3 (orb6881) at 1:300 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 57kD, Observed band size: 57kD.
Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human Raji cell lysates, Primary: Anti-RIPK3 (orb6881) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kDa, Observed band size: 60 kDa.
Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-RIPK3 (orb6881) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.
Tissue/Cell: human pneumonia tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-RIPK3 Polyclonal Antibody, Unconjugated (orb6881) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Western blot:A549 cells,Primary:Anti-RIPK3 at 1:300 dilution.
Western blot:Spleen (Mouse),Primary:Anti-RIPK3 at 1:1000 dilution.



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