Phospho-Smad1/5 (Ser463 + Ser465) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6977
Article Name: Phospho-Smad1/5 (Ser463 + Ser465) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6977
Supplier Catalog Number: orb6977
Alternative Catalog Number: BYT-ORB6977-50,BYT-ORB6977-100,BYT-ORB6977-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad1/5 around the phosphorylation site of Ser463/465 IS(p-S)V(p-S)
Conjugation: Unconjugated
Alternative Names: SMAD1 & SMAD5 (p-S463/S465), p-SMAD1 & SMAD5, phospho-SMAD1 & SMAD5, BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, Mad1, Mlp1, MusMLP, dwf-A, mMad1, SMAD1_HUMAN, SMAD1, MAD homolog 1, Mothers against DPP homolog 1, Mad-related protein 1, SMAD family member 1 (SMAD 1 | Smad1 | hSMAD1), Transforming growth factor-beta-signaling protein 1 (BSP-1), SMAD1_MOUSE, Dwarfin-A (Dwf-A), Mothers-against-DPP-related 1 (Mad-related protein 1 | mMad1), SMAD family member 1 (SMAD 1 | Smad1), SMAD1_RAT,
Phospho-Smad1/5 (Ser463 + Ser465) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 52 kDa
UniProt: Q15797
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SMAD1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Application Notes: Modification: Phosphorylated
Cerebrum (Mouse) Lysate at 40 ug, Stomach (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) (orb6977) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 62 kD.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated (orb6977) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Smad1-5 (Ser463+ Ser465)) Polyclonal Antibody, Unconjugated (orb6977) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Skin (Mouse) Lysate at 40 ug, Lane 5: Kidney (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Rat) Lysate at 40 ug, Lane 7: Testis (Rat) Lysate at 40 ug, Lane 8: Skin (Rat) Lysate at 40 ug, Lane 9: Kidney (Rat) Lysate at 40 ug, Lane 10: Huvec (Human) Cell Lysate at 30 ug, Lane 11: A549 (Human) Cell Lysate at 30 ug, Lane 12: Hela (Human) Cell Lysate at 30 ug, Lane 13: HT1080 (Human) Cell Lysate at 30 ug, Lane 14: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Smad3 (Ser463 + Ser465) (orb6977) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 54 kD.
Western blot analysis of mouse intestinal lysate using Smad1 (phospho-Ser463-465) antibody.
Western blot analysis of mouse stomach lysate using Smad1 (phospho-Ser463-465) antibody.



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