Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB6984
- Images (6)
| Article Name: | Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB6984 |
| Supplier Catalog Number: | orb6984 |
| Alternative Catalog Number: | BYT-ORB6984-50,BYT-ORB6984-100,BYT-ORB6984-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser425 SV(p-S)-NH2 |
| Conjugation: | Unconjugated |
| Alternative Names: | SMAD3 (p-S425), p-SMAD3, phospho-SMAD3, HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3, Smad 3, SMAD3_HUMAN, SMAD3, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3 (SMAD 3 | Smad3 | hSMAD3), SMAD3_MOUSE, mMad3, SMAD family member 3 (SMAD 3 | Smad3), SMAD3_RAT, |
| Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 47 kDa |
| UniProt: | P84022 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SMAD3 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
| Application Notes: | Modification: Phosphorylated |
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Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-Phospho-Smad3 (Ser425) antibody (orb6984), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Smad3 (Ser425)) Polyclonal Antibody, Unconjugated (orb6984) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Smad3 (Ser425)) Polyclonal Antibody, Unconjugated (orb6984) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Smad3 (Ser425)) Polyclonal Antibody, Unconjugated (orb6984) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Phospho-Smad3 (Ser425) (orb6984) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 50 kD. |
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Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-Smad3 (Ser425) Polyclonal Antibody, Unconjugated (orb6984) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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