SPHK2 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB7003
Article Name: SPHK2 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB7003
Supplier Catalog Number: orb7003
Alternative Catalog Number: BYT-ORB7003-50,BYT-ORB7003-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human SPHK2 (101-200/654aa)
Conjugation: Unconjugated
Alternative Names: SK 2, SK-2, SPK 2, SPK-2, Sk2, Spk2, SPHK2_HUMAN, SPHK2, 2.7.1.91, SPHK2_MOUSE,
SPHK2 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 72 kDa
UniProt: Q9NRA0
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SPHK2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SPHK2) Polyclonal Antibody, Unconjugated (orb7003) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Human SH-SY5Y cell Lysates, Lane 2: Human A549 cell Lysates, Lane 3: Human HepG2 cell Lysates, Primary: Anti-SPHK2 (orb7003) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72kDa, Observed band size: 72kDa.
Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-SPHK2 (orb7003) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72kD, Observed band size: 75kD.
Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-SPHK2 (orb7003) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72kD, Observed band size: 75kD.
SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (SPHK2) polyclonal Antibody, Unconjugated (orb7003) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
IHC-P analysis of mouse brain tissue using SPHK2 Polyclonal Antibody at 1:400 dilution.


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