Synapsin 1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB7040
Article Name: Synapsin 1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB7040
Supplier Catalog Number: orb7040
Alternative Catalog Number: BYT-ORB7040-50,BYT-ORB7040-100,BYT-ORB7040-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Synapsin I (201-300/705aa)
Conjugation: Unconjugated
Alternative Names: EPILX, EPILX1, MRX50, SYN1a, SYN1b, SYNI, Syn-1, Syn1-S, SYN1_HUMAN, SYN1, Brain protein 4.1, Synapsin I, SYN1_MOUSE, SYN1_RAT,
Synapsin 1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 85 kDa
UniProt: P17600
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SYN1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Synapsin 1) Polyclonal Antibody, Unconjugated (orb7040) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Synapsin 1) Polyclonal Antibody, Unconjugated (orb7040) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: A549 (Human) Cell Lysate at 40 ug, Primary: Anti-Synapsin 1 (orb7040) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.
Sample: HL-60 (Human) Cell Lysate at 40 ug, Primary: Anti-Synapsin 1 (orb7040) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.
Immunohistochemical staining of rat brain tissue using Synapsin I antibody.
Western blot analysis of extracts from Hl-60 cell using Synapsin antibody.



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