GLI1 Recombinant Rabbit Monoclonal Antibody, Clone: [5C3], Unconjugated
Catalog Number:
BYT-ORB704320
- Images (6)
| Article Name: | GLI1 Recombinant Rabbit Monoclonal Antibody, Clone: [5C3], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704320 |
| Supplier Catalog Number: | orb704320 |
| Alternative Catalog Number: | BYT-ORB704320-50,BYT-ORB704320-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant human GLI1 |
| Conjugation: | Unconjugated |
| Alternative Names: | GLI1_HUMAN, GLI1, Glioma-associated oncogene, Oncogene GLI, GLI, GLI1_MOUSE, Glioma-associated oncogene homolog, |
| GLI1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [5C3] |
| Molecular Weight: | 118 kDa |
| UniProt: | P08151 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GLI1 |
| Application Dilute: | WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
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293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Gli1) monoclonal Antibody, Unconjugated (orb704320) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Gli1) monoclonal Antibody, Unconjugated (orb704320) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (mouse fallopian tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLI1) Monoclonal Antibody, Unconjugated (orb704320) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLI1) Monoclonal Antibody, Unconjugated (orb704320) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: K562 cell lysate, Lane 2: SH-SY5Y cell lysate, Primary: Anti-GLI1 (orb704320) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 118 kD, Observed band size: 145 kD. |
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SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Gli1) monoclonal Antibody, Unconjugated (orb704320) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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