CYP2E1 Recombinant Rabbit Monoclonal Antibody, Clone: [1A26], Unconjugated
Catalog Number:
BYT-ORB704324
- Images (6)
| Article Name: | CYP2E1 Recombinant Rabbit Monoclonal Antibody, Clone: [1A26], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704324 |
| Supplier Catalog Number: | orb704324 |
| Alternative Catalog Number: | BYT-ORB704324-50,BYT-ORB704324-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human CYP2E1 |
| Conjugation: | Unconjugated |
| Alternative Names: | CPE1, CYP2E, P450-J, P450C2E, CYPIIE1, CP2E1_HUMAN, CYP2E1, 4-nitrophenol 2-hydroxylase, Cytochrome P450-J, 1.14.14.1, CP2E1_MOUSE, Cytochrome P450-ALC, Cyp2e-1, CP2E1_RABIT, Cytochrome P450 isozyme 3A (Cytochrome P450 LM3A), CP2E1_RAT, Cytochrome P450RLM6, |
| CYP2E1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [1A26] |
| Molecular Weight: | 57 kDa |
| UniProt: | P05181 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CYP2E1 |
| Application Dilute: | WB=1:500-2000, ICC/IF=1:50-200 |
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293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (CYP2E1) monoclonal Antibody, Unconjugated (orb704324) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (CYP2E1) monoclonal Antibody, Unconjugated (orb704324) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (orb704324) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (orb704324) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (orb704324) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: human liver tissue lysates, Primary: Anti-CYP2E1 (orb704324) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD. |
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