0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
CR2
Application Dilute:
WB=1:500-2000, Flow-Cyt=1µg/Test
Flow cytometric analysis of Molt-4 Cells using CD21 antibody.
Immunohistochemical staining of human lung carcinoma tissue using CD21 antibody.
Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (orb7143/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.
Blank control (blue): U-87MG Cells (fixed with 2% paraformaldehyde (10 min). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (orb7143/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.
Blank control: Raji cells (blue). Primary Antibody: Rabbit Anti-CD21 antibody (orb7143), dilution: 1 µg in 100 1 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-CD21 (orb7143) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 111 kD, Observed band size: 111 kD.
* VAT and and shipping costs not included. Errors and price changes excepted
