Clathrin heavy chain/CLTC Mouse Monoclonal Antibody, Clone: [6D3], Unconjugated
Biozol Catalog Number:
BYT-ORB738492
Supplier Catalog Number:
orb738492
Alternative Catalog Number:
BYT-ORB738492-100
Manufacturer:
Biorbyt
Host:
Mouse
Category:
Antikörper
Application:
FC, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
E.coli-derived human Clathrin heavy chain/CLTC recombinant protein (Position: R967-Q1668).
Conjugation:
Unconjugated
Alternative Names:
CHC, CHC17, Clathrin heavy chain 1, clathrin, heavy chain (Hc), CLH 17, CLH17, CLTC, CLTCL2, Hc, KIAA0034
Anti-Clathrin heavy chain/CLTC Antibody (monoclonal, 6D3). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Form:
Lyophilized
Target:
Clathrin heavy chain 1
Application Dilute:
Western blot, 0.25-0.5µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x10 6 cells, Human
Flow Cytometry analysis of HepG2 cells using anti-Clathrin heavy chain/CLTC antibody. Overlay histogram showing HepG2 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Clathrin heavy chain/CLTC Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-mouse IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IHC analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody. Clathrin heavy chain/CLTC was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Clathrin heavy chain/CLTC Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody. Clathrin heavy chain/CLTC was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Clathrin heavy chain/CLTC Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody. Clathrin heavy chain/CLTC was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Clathrin heavy chain/CLTC Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Clathrin heavy chain/CLTC antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Clathrin heavy chain/CLTC at approximately 180 KD. The expected band size for Clathrin heavy chain/CLTC is at 180 KD.
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