Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Form:
Lyophilized
Target:
Interleukin-1 receptor antagonist protein
Application Dilute:
Western blot, 0.25-0.5µg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3µg/1x10 6 cells, Mouse, Rat ELISA, 0.1-0.5µg/ml, -
Flow Cytometry analysis of HEPA1-6 cells using anti-IL1RA/Il1rn antibody. Overlay histogram showing HEPA1-6 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of NRK cells using anti-IL1RA/Il1rn antibody. Overlay histogram showing NRK cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IHC analysis of IL1RA/Il1rn using anti-IL1RA/Il1rn antibody. IL1RA/Il1rn was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL1RA/Il1rn Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of IL1RA/Il1rn using anti-IL1RA/Il1rn antibody. IL1RA/Il1rn was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL1RA/Il1rn Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Western blot analysis of IL1RA/Il1rn using anti-IL1RA/Il1rn antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA/Il1rn antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RA/Il1rn at approximately 18 KD. The expected band size for IL1RA/Il1rn is at 18 KD.
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