Recombinant human protein was used as the immunogen for the SUMO-2 antibody.
Conjugation:
Unconjugated
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer s disease.
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Application Notes: Optimal dilution of the SUMO-2 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-2 antibody (SUMO2/1199)
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-2 antibody.
IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with SUMO-2 antibody.
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with SUMO-2 antibody.
IHC: Formalin-fixed, paraffin-embedded rat ovary stained with SUMO-2 antibody.
IHC: Formalin-fixed, paraffin-embedded rat heart stained with SUMO-2 antibody.
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