Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1 µg/ml (clone: DM59) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry data of serially titrated Rabbit anti-CD27 monoclonal antibody (clone: DM59) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of rabbit anti-human CD27 mAb (orb757382). (A) orb757382 does not bind to 293T cells that do not express CD27. (B) A clear peak shift of orb757382 was seen compared to the control when incubated with CD27-expressing Raji cells, indicating strong binding of orb757382 to CD27. Antibodies were incubated at 2 µg/mL.
Anti-CD27 antibody (SKU orb757382) at 1/1000 dilution.Lane: Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 29 kDa.Observed band size: 55 kDa
A. orb757382 at 10 µg/ml staining CD27 in Raji cells by IHC (SKU orb757382), B. orb757382 at 10 µg/ml staining CD27 in human tonsil tissue by IHC (SKU orb757382).
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