ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human MSLN protein, mFc-His tagged protein orb689392 can bind Rabbit anti-MSLN monoclonal antibody (clone: DM73) in a linear range of 1-100 ng/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1 µg/ml (clone: DM73) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody (clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of rabbit anti-human Mesothelin mAb (orb757396). (A) orb757396 does not bind to 293T cells that do not express Mesothelin. (B) A clear peak shift of orb757396 was seen compared to the control when incubated with Mesothelin-expressing Hela cells, indicating strong binding of orb757396 to Mesothelin. Antibodies were incubated at 2 µg/mL.
Anti-MSLN antibody (SKU orb757396) at 1/1000 dilution.Lane: HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 69 kDa.Observed band size: 50 kDa
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