Phospho-AKT1 (Ser124) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB783188
- Images (7)
| Article Name: | Phospho-AKT1 (Ser124) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB783188 |
| Supplier Catalog Number: | orb783188 |
| Alternative Catalog Number: | BYT-ORB783188-50,BYT-ORB783188-100,BYT-ORB783188-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Ser124 SG(p-S)PS |
| Conjugation: | Unconjugated |
| Alternative Names: | AKT1 (p-S124), p-AKT1, phospho-AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, LTR-akt, PKB/Akt, PKBalpha, O57513_CHICK, akt1, RAC-PK-alpha, 2.7.11.1, AKT1_HUMAN, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, AKT1_MOUSE, AKT1 kinase, Thymoma viral proto-oncogene, AKT1_RAT, |
| Phospho-AKT1 (Ser124) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 60 kDa |
| UniProt: | P31749 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | AKT1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser124)) Polyclonal Antibody, Unconjugated (orb783188) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser124)) Polyclonal Antibody, Unconjugated (orb783188) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser124)) Polyclonal Antibody, Unconjugated (orb783188) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser124)) Polyclonal Antibody, Unconjugated (orb783188) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 (Ser124)) Polyclonal Antibody, Unconjugated (orb783188) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Heart tissue lysates, Primary: Anti-phospho-AKT1 (Ser124) (orb783188) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD. |
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IHC-P analysis of rat lung using phospho-AKT1 Ser124 Polyclonal Antibody at 1:200 dilution. |
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