Phospho-MARK2 (Ser595) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB783419
Article Name: Phospho-MARK2 (Ser595) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB783419
Supplier Catalog Number: orb783419
Alternative Catalog Number: BYT-ORB783419-50,BYT-ORB783419-100,BYT-ORB783419-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human MARK2 around the phosphorylation site of Ser595 SR(p-S)TF
Conjugation: Unconjugated
Alternative Names: MARK2 (p-S595), p-MARK2, phospho-MARK2, EMK-1, EMK1, MRD76, PAR-1, Par-1b, Par1b, Emk, MARK2_HUMAN, MARK2, ELKL motif kinase 1 (EMK-1), MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b (Par-1b | Par1b), 2.7.11.1, 2.7.11.26, MARK2_MOUSE, PAR1 homolog b (Par-1b | mPar-1b), MARK2_RAT,
Phospho-MARK2 (Ser595) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 82-90 kDa
UniProt: Q7KZI7
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MARK2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MARK2 (Ser595)) Polyclonal Antibody, Unconjugated (orb783419) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MARK2 (Ser595)) Polyclonal Antibody, Unconjugated (orb783419) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-MARK2 (Ser595)) Polyclonal Antibody, Unconjugated (orb783419) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-phospho-MARK2 (Ser595) (orb783419) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 82-90kD Observed band size: 90kD.
Sample: Siha Cell (Human) Lysate at 30 ug Primary: Anti-phospho-MARK2 (Ser595) (orb783419) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 82-90kD Observed band size: 90kD.
IHC-P analysis of Rat liver using p-MARK2 Ser595 Polyclonal Antibody at 1:400 dilution.
IHC-P analysis of Human kidney using phospho-MARK2 Ser595 Polyclonal Antibody at 1:200 dilution.



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