LXR alpha Recombinant Rabbit Monoclonal Antibody, Clone: [4A8], Unconjugated
Catalog Number:
BYT-ORB783426
- Images (7)
| Article Name: | LXR alpha Recombinant Rabbit Monoclonal Antibody, Clone: [4A8], Unconjugated |
| Biozol Catalog Number: | BYT-ORB783426 |
| Supplier Catalog Number: | orb783426 |
| Alternative Catalog Number: | BYT-ORB783426-50,BYT-ORB783426-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human LXR alpha (60-110/447aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | LXR-a, LXRA, RLD-1, LXR, RLD1, Unr1, LXRalpha, NR1H3_HUMAN, NR1H3, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3, NR1H3_MOUSE, NR1H3_RAT, |
| LXR alpha Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [4A8] |
| Molecular Weight: | 50 kDa |
| UniProt: | Q13133 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | NR1H3 |
| Application Dilute: | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100 |
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Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-LXR alpha antibody (orb783426), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (LXR alpha) monoclonal Antibody, Unconjugated (orb783426) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human placenta tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LXR alpha) Monoclonal Antibody, Unconjugated (orb783426) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human spleen tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LXR alpha) Monoclonal Antibody, Unconjugated (orb783426) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LXR alpha) Monoclonal Antibody, Unconjugated (orb783426) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: human liver tissue lysates, Primary: Anti-LXR alpha (orb783426) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 50 kD, Observed band size: 53 kD. |
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Sample: Lane 1: mouse colon tissue lysates, Primary: Anti-LXR alpha (orb783426) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD. |
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