Ly6a Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting Ly6a. This antibody is suitable for FC, IF. It exhibits reactivity with Mouse, Rat samples.
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Target:
Ly6a
Application Dilute:
Flow-Cyt=2µg /Test, IF=1:100-500
Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-Sca1/FITC Conjugated antibody (orb9766), dilution: 0.2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Mouse thymus. Primary Antibody (green line): Rabbit Anti-Sca1 antibody (orb9766), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Rat spleen. Primary Antibody (green line): Rabbit Anti-Sca1 antibody (orb9766), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Immunohistochemical staining of rat kidney tissue using Sca1 antibody (FITC) (Antibody)
Immunofluorescence analysis of mouse thymus tissue using Sca1 antibody (FITC) (Antibody)
Flow cytometric analysis of Mouse Thymus Cells using Sca1 antibody (FITC) (An
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