Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB99303
- Images (7)
| Article Name: | Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB99303 |
| Supplier Catalog Number: | orb99303 |
| Alternative Catalog Number: | BYT-ORB99303-50,BYT-ORB99303-100,BYT-ORB99303-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 2 around the phosphorylation site of Thr172 LR(p-T)SC |
| Conjugation: | Unconjugated |
| Alternative Names: | PRKAA2 | AMPK alpha-2 (p-T172), p-AMPK alpha-2, phospho-AMPK alpha-2, AMPK, AMPK2, AMPKa2, PRKAA, 2310008I11Rik, A830082D05, AMPKalpha2, AAPK2_HUMAN, PRKAA2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase (ACACA kinase), Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase), 2.7.11.1, AAPK2_MOUSE, AAPK2_RAT, |
| Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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IHC-P analysis of Rat testis using phospho |
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