SLC2A3 Recombinant Monoclonal Antibody, Clone: [14B1], Unconjugated, Rabbit
Catalog Number:
CSB-RA162667A0HU
- Images (5)
| Article Name: | SLC2A3 Recombinant Monoclonal Antibody, Clone: [14B1], Unconjugated, Rabbit |
| Biozol Catalog Number: | CSB-RA162667A0HU |
| Supplier Catalog Number: | CSB-RA162667A0HU |
| Alternative Catalog Number: | CSB-RA162667A0HU-100UL, CSB-RA162667A0HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, FC, IHC, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | Solute carrier family 2, facilitated glucose transporter member 3 (Glucose transporter type 3, brain) (GLUT-3) , SLC2A3, GLUT3 |
| Clonality: | Monoclonal |
| Clone Designation: | [14B1] |
| UniProt: | P11169 |
| Buffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Purity: | Affinity-chromatography |
| Form: | Liquid |
| Target: | SLC2A3 |
| Antibody Type: | Recombinant Antibody |
| Application Dilute: | Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, FC:1:50-1:200 |
|
|
Overlay Peak curve showing MCF7 cells stained with CSB-RA162667A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
|
|
IHC image of CSB-RA162667A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
|
|
IHC image of CSB-RA162667A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
|
|
IHC image of CSB-RA162667A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
|
|
Western Blot Positive WB detected in: 293 whole cell lysate (20µg) , JK whole cell lysate (20µg) , Hela whole cell lysate (20µg) , HepG2 whole cell lysate (20µg) , SY5Y whole cell lysate (20µg) All lanes: SLC2A3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa |
Product Guarantee and Expert Support
