CELF1 Recombinant Monoclonal Antibody, Clone: [1G2], Unconjugated, Rabbit
Catalog Number:
CSB-RA561537A0HU
- Images (5)
| Article Name: | CELF1 Recombinant Monoclonal Antibody, Clone: [1G2], Unconjugated, Rabbit |
| Biozol Catalog Number: | CSB-RA561537A0HU |
| Supplier Catalog Number: | CSB-RA561537A0HU |
| Alternative Catalog Number: | CSB-RA561537A0HU-100UL, CSB-RA561537A0HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | 50 kDa Nuclear polyadenylated RNA binding protein antibody, 50 kDa nuclear polyadenylated RNA-binding protein antibody, Bruno like 2 antibody, bruno like protein 2 antibody, Bruno-like protein 2 antibody, BRUNOL 2 antibody, BRUNOL2 antibody, CELF 1 antibody, CELF-1 antibody, celf1 antibody, CELF1 CUGBP, Elav like family member 1 antibody, CELF1_HUMAN antibody, CUG BP and ETR 3 like factor 1 antibody, CUG BP antibody, CUG BP1 antibody, CUG RNA binding protein antibody, CUG triplet repeat RNA binding protein 1 antibody, CUG triplet repeat RNA-binding protein 1 antibody, CUG-BP antibody, CUG-BP- and ETR-3-like factor 1 antibody, CUG-BP1 antibody, CUGBP 1 antibody, CUGBP and ETR3 like factor 1 antibody, CUGBP antibody, CUGBP Elav like family member 1 antibody, CUGBP Elav-like family member 1 antibody, CUGBP1 antibody, Cytidine uridine guanosine binding protein 1 antibody, Deadenylation factor CUG BP antibody, Deadenylation factor CUG-BP antibody, Deadenylation factor CUGBP antibody, EDEN BP antibody, EDEN BP homolog antibody, EDEN-BP antibody, EDEN-BP homolog antibody, embryo deadenylation element binding protein antibody, embryo deadenylation element binding protein homolog antibody, Embryo deadenylation element-binding protein homolog antibody, hNab 50 antibody, hNab50 antibody, NAB 50 antibody, NAB50 antibody, NAPOR antibody, Nuclear polyadenylated RNA binding protein 50 kD antibody, Nuclear polyadenylated RNA binding protein antibody, RNA binding protein BRUNOL 2 antibody, RNA binding protein BRUNOL2 antibody, RNA-binding protein BRUNOL-2 antibody |
| Clonality: | Monoclonal |
| Clone Designation: | [1G2] |
| UniProt: | Q92879 |
| Buffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Purity: | Affinity-chromatography |
| Form: | Liquid |
| Target: | CELF1 |
| Antibody Type: | Recombinant Antibody |
| Application Dilute: | Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
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Overlay Peak curve showing Hela cells stained with CSB-RA561537A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of Hela cell with CSB-RA561537A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) . |
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IHC image of CSB-RA561537A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-RA561537A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: U-937 whole cell lysate(20µg) ,U-251MG whole cell lysate(20µg) , SH-SY5Y whole cell lysate(20µg) , A549 whole cell lysate(20µg) ,HepG2 whole cell lysate(20µg) ,NIH/3T3 whole cell lysate(20µg) ,Hela whole cell lysate(20µg) All lanes: CELF 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time:1min20s |
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