PARP1 Recombinant Monoclonal Antibody, Clone: [8C7], Unconjugated, Rabbit
Catalog Number:
CSB-RA581794A0HU
- Images (5)
| Article Name: | PARP1 Recombinant Monoclonal Antibody, Clone: [8C7], Unconjugated, Rabbit |
| Biozol Catalog Number: | CSB-RA581794A0HU |
| Supplier Catalog Number: | CSB-RA581794A0HU |
| Alternative Catalog Number: | CSB-RA581794A0HU-100UL, CSB-RA581794A0HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IHC, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | Poly [ADP-ribose] polymerase 1 (PARP-1) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 1) (ARTD1) (NAD(+) ADP-ribosyltransferase 1) (ADPRT 1) (Poly[ADP-ribose] synthase 1) , PARP1, ADPRT PPOL |
| Clonality: | Monoclonal |
| Clone Designation: | [8C7] |
| UniProt: | P09874 |
| Buffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Purity: | Affinity-chromatography |
| Form: | Liquid |
| Target: | PARP1 |
| Antibody Type: | Recombinant Antibody |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, FC:1:20-1:200 |
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Overlay histogram showing Jurkat cells stained with CSB-RA581794A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of Hela Cells with CSB-RA581794A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) . |
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IHC image of CSB-RA581794A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-RA581794A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: K562 whole cell lysate All lanes: PARP antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 114 KDa Observed band size: 114 kDa |
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