ERK 8 (phospho Thr175/Y177) rabbit pAb, Rabbit, Polyclonal

Article Name:	ERK 8 (phospho Thr175/Y177) rabbit pAb, Rabbit, Polyclonal
Biozol Catalog Number:	EBT-ES5241
Supplier Catalog Number:	ES5241
Alternative Catalog Number:	EBT-ES5241-50, EBT-ES5241-100
Manufacturer:	ELK Biotechnology
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC, WB
Species Reactivity:	Human, Mouse
Immunogen:	The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177. AA range:141-190
Alternative Names:	MAPK15, ERK7, ERK8, Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.,function:In vitro, phosphorylates MBP.,PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1.,tissue specificity:Widely expressed with a maximal expression in lung and kidney.,

Clonality:	Polyclonal
Concentration:	1 mg/ml
Molecular Weight:	60kD
NCBI:	225689
UniProt:	Q8TD08

Application Dilute:

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

	Western Blot analysis of Jurkat cells using Phospho-ERK 8 (T175/Y177) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
	Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
	Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
	Western blot analysis of lysates from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) Antibody. The lane on the right is blocked with the phospho peptide.