Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was a
Fibronectin antibody LS-B382 is an unconjugated rabbit polyclonal antibody to human Fibronectin (FN1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Immunohistochemistry: LS-B382 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,
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