Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE PPAT antibody diluted at 1:1000
Immunofluorescence of methanol-fixed HeLa, using PPAT antibody at 1:500 dilution.
* VAT and and shipping costs not included. Errors and price changes excepted