Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE YARS2 antibody diluted at 1:3000
Immunofluorescence of methanol-fixed HeLa, using YARS2 antibody at 1:500 dilution.
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