A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4.
Conjugation:
Unconjugated
Histone H4 antibody LS-C335247 is an unconjugated rabbit polyclonal antibody to Histone H4 (Asym-Di-Methyl-Arg3) from human. It is reactive with human, mouse and rat. Validated for DB, IF, IHC, IP and WB.
Clonality:
Polyclonal
Buffer:
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Purity:
Affinity purified
Form:
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Application Dilute:
DB, IF (1:50 - 1:200), IHC (1:50 - 1:200), IP (1:50 - 1:100), WB (1:500 - 1:2000)
Application Notes:
The predicted MW is 11kDa, while the observed MW by Western blot was 13kDa.
Western blot analysis of extracts of HeLa cells and H4 protein expressed in E.coli.
Immunohistochemistry of paraffin-embedded rat brain tissue.
Immunofluorescence analysis of 293T cells.
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