A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3.
Conjugation:
Unconjugated
Histone H3 antibody LS-C335322 is an unconjugated rabbit polyclonal antibody to Histone H3 (Sym-Di-Methyl-Arg26) from human. It is reactive with human, mouse and rat. Validated for DB, IF and WB.
Clonality:
Polyclonal
Buffer:
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Purity:
Affinity purified
Form:
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Application Dilute:
DB, IF (1:50 - 1:200), WB (1:500 - 1:2000)
Application Notes:
The predicted MW is 15kDa, while the observed MW by Western blot was 17kDa.
Western blot analysis of extracts of HeLa cells and H3 protein expressed in E.coli.
Dot-blot analysis of all sorts of methylation peptides.
Immunofluorescence analysis of 293T cells.
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