ELISA (1:200), IF (1:80), IHC-P (1:500 - 1:1000), RIA
Application Notes:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solutio
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