30 kDa splicing factor SMNrp, MGC106917, MGC112663, SMN related protein, SMNR, SPF30, Splicing factor 30, survival of motor neuron-related, Survival motor neuron domain containing protein 1, Survival of motor neuron related splicing factor 30
SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (survival motor neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunofluorescence Analysis of PFA-fixed HeLa cells using SMNDC1 Mouse Monoclonal An
Flow Cytometric Analysis of PFA-fixed HeLa cells. SMNDC1 Mouse Monoclonal Antibody (PCRP-SMNDC1-1A9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells using SMNDC1 Mouse Monoclonal Antibody (PCRP-SMNDC1-1A9) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using SMNDC1 Mouse Monoclonal Antibody (PCRP-SMNDC1-1A9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
SDS-PAGE Analysis. Purified SMNDC1 Mouse Monoclonal Antibody (PCRP-SMNDC1-1A9). Confirmation of Purity and Integrity of Antibody.
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