Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM ED
Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).
SDS-PAGE Analysis of Purified CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 / CR2 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
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