Recognizes a single chain glycoprotein of 70kDa, identified as CD55. This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Optimal dilution for a specific application should be determined.
Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55 M
Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Green). Counterstain is RedDot.
Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)
SDS-PAGE Analysis of Purified CD55 Mouse Monoclonal Antibody (F4-29D9) . Confirmation of Integrity and Purity of Antibody.
* VAT and and shipping costs not included. Errors and price changes excepted
