Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
Western Blot Analysis of human skin lysate using HER-2 Rabbit Recombinant Monoclonal Antibody (ERBB2/8142R).
Western Blot Analysis of skin tissue lysates of different species using HER-2 Rabbit Recombinant Monoclonal Antibody (ERBB2/8142R).
SDS-PAGE Analysis of Purified CD340 Recombinant Rabbit Monoclonal Antibody (ERBB2/8142R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human breast carcinoma stained with HER-2 Recombinant Rabbit Monoclonal Antibody (ERBB2/8142R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human breast carcinoma stained with HER-2 Recombinant Rabbit Monoclonal Antibody (ERBB2/8142R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
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