Recombinant fragment corresponding to C-terminus of human ER-alpha
Alternative Names:
Estrogen Receptor alpha delta 4*5,6,7*/654 isoform, Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1
Recognizes a protein of 67kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.
200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 2
Formalin fixed paraffin embedded human breast carcinoma
Formalin fixed paraffin embedded human breast carcinoma stained with ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/6811R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Formalin fixed paraffin embedded human breast carcinoma stained with ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/6811R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Western blot analysis of MCF-7 cell lysate using ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/6811R).
SDS-PAGE Analysis of Purified ESR1 Recombinant Rabbit Monoclonal Antibody(ESR1/6811R). Confirmation of Purity and Integrity of Antibody.
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