75SirT1, hSIR2, hSIRT1, NAD dependent deacetylase sirtuin 1, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2 like protein 1, SIR2, S.cerevisiae, homolog-like 1, SIR2ALPHA, SIR2L1, SirtT1 75 kDa fragment, Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), Sirtuin type 1
The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent matingtype loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)), ,Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Optimal dilution for a spec
SDS-PAGE Analysis of Purified SIRT1 Mouse Monoclonal Antibody (PCRP-SIRT1-1E11). Confi
Flow cytometric analysis of PFA-fixed HeLa cells. SIRT1Mouse Monoclonal Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis of Purified SIRT1 Mouse Monoclonal Antibody (PCRP-SIRT1-1E11). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SIRT1 Mouse Monoclonal Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using SIRT1-Monospecific Mouse Monoclonal Antibody (PCRP-SIRT1-1E11). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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