This antibody recognizes a protein of 42kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 min
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR / p504S Mouse Monoclonal Antibody (AMACR/1723).
SDS-PAGE Analysis Purified AMACR / p504S Mouse Monoclonal Antibody (AMACR/1723). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of PC3 cell lysate using Purified AMACR / p504S Mouse Monoclonal Antibody (AMACR/1723).
Western Blot Analysis of Liver tissue lysate using Purified AMACR / p504S Mouse Monoclonal Antibody (AMACR/1723).
Western Blot analysis of Kidney tissue lysate using AMACR Mouse Monoclonal Antibody (AMACR/1723).
Western blot analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney and Guinea pig Kidney tissue lysates using AMACR Mouse Monoclonal Antibody (AMACR/1723).
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