CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°
FACS analysis of CD15 on human Monocytes using CD15 Monoclonal Antibody (SPM119).
Formalin-fixed, paraffin-embedded human Hodgkin&apos,s Lymphoma stained with CD15 Monoclonal Antibody (SPM119).
FACS analysis of CD15 on human Monocytes using CD15 Monoclonal Antibody (SPM119).
Immunofluorescence staining of U937 cells using CD15 Monoclonal Antibody (SPM119) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Flow Cytometric Analysis of U937 cells using CD15 MAb (SPM119) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
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