CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)), ,Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Optimal dilution for a spec
SDS-PAGE Analysis of Purified CNOT10 Mouse Monoclonal Antibody (PCRP-CNOT10-1D5). Co
Flow cytometric analysis of PFA-fixed HeLa cells. CNOT10 Mouse Monoclonal Antibody (PCRP-CNOT10-1D5) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis of Purified CNOT10 Mouse Monoclonal Antibody (PCRP-CNOT10-1D5). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using CNOT10 Mouse Monoclonal Antibody (PCRP-CNOT10-1D5)
followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
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