GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
SDS-PAGE Analysis Purified GATA-3 Mouse Monoclonal A
Formalin-fixed, paraffin-embedded human lobular breast carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/6664) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis Purified GATA-3 Mouse Monoclonal Antibody (GATA3/6664). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human lobular breast carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/6664).
Formalin-fixed, paraffin-embedded human bladder stained with GATA-3 Mouse Monoclonal Antibody (GATA3/6664).
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using GATA3/6664 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
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