Bovine, Gallus, Human, Mouse, Porcine, Rabbit, Rat
Immunogen:
Recombinant GFAP protein
Alternative Names:
Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 2
Formalin-fixed, paraffin-embedded Rat C
Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP Mouse Monoclonal Antibody (ASTRO/789).
Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Mouse Monoclonal Antibody (ASTRO/789).
Formalin-fixed, paraffin-embedded Rat Cerebellum stained with GFAP Mouse Monoclonal Antibody (ASTRO/789).
Western Blot Analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (ASTRO/789).
SDS-PAGE Analysis Purified GFAP Mouse Monoclonal Antibody (ASTRO/789). Confirmation of Integrity and Purity of Antibody.
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