GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 2
Formalin-fixed, par
SDS-PAGE Analysis of Purified GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).
Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).
Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).
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