PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA),,Flow Cytometry (1-2ug/ml),,Immunofluorescence (1-2ug/ml),,Optimal dilution for a specific application should be determined.
Flow Cytometric Analysis of human Jurkat cells using
Immunofluorescence Analysis of human Jurkat cells labeling PD-L1 with PD-L1 Mouse Monoclonal Antibody (PDL1/2743) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of human Jurkat cells using PD-L1 Mouse Monoclonal Antibody (PDL1/2743) followed by Goat anti-Mouse IgG-CF488 (Orange), cells alone (Blue), Isotype Control (Red).
SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2743). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1 Mouse Monoclonal Antibody (PDL1/2743). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD,Aövivs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD,Aövivs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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