Recombinant fragment (around aa 374-540) of human MSH6 protein (exact sequence is proprietary)
Alternative Names:
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160kDa subunit, MutS-alpha 160kDa subunit, p160, Sperm associated protein
The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
ELISA (For coating, order Ab without BSA), Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
Formalin-fixed, paraffin-embedded human colon stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592R).
Western blot analysis of HCT-116 cell lysate using MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592).
SDS-PAGE Analysis Purified MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of DU 145 lysate using MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592R).
Western Blot Analysis of HEK293 cell lysate using MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592R).
