Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)
Alternative Names:
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160kDa subunit, MutS-alpha 160kDa subunit, p160, Sperm associated protein
MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI). MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsiblefor the MSI.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
ELISA (For coating, order Ab without BSA), Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
Western blot analysis of HCT-116 cell lysate using MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R).
Formalin-fixed, paraffin-embedded human colon from Lynch Disease stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R).
SDS-PAGE Analysis of Purified MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human esophagus stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/6654R). Inset: PBS instead of primary antibody, secondary only negative control.
