Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface nonself antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin s Disease.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
Western
Formalin-fixed, paraffin-embedded human spleen stained with Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).
Formalin-fixed, paraffin-embedded human tonsil stained with Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).
Western Blot Analysis of human spleen tissue lysate using Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).
SDS-PAGE Analysis of Purified GZMB Recombinant Mouse Monoclonal Antibody (rGZMB/4538). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Kidney, Human Lung and Human Spleen tissue lysates using Granzyme B Recombinant Mouse Monoclonal Antibody (rGZMB/4538).
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