It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming (TM)-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of (TM)-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Immunofluorescence Analys
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).
Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. IDH1 Mouse Monoclonal Antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.
Western Blot Analysis of A-549 lysate using IDH1 Mouse Monoclonal Antibody (IDH1/1152).
Western Blot Analysis of Human Kidney tissue lysate using IDH1 Mouse Monoclonal Antibody (IDH1/1152).
* VAT and and shipping costs not included. Errors and price changes excepted
