It recognizes a protein of 80-87kDa, identified as gamma-catenin. The catenins ((TM), A°a, A and OOL) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. (TM) T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. (TM) T-catenin binds to (TM) E-catenin as well as to A°a-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for (TM)-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), ,Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues req
Western blot analysis: HT20, 293, A431, MCF-7, HepG2 & A549 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Catenin, gamma Mouse Monoclonal Antibody (15F11) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot (red).
Western blot analysis of HeLa and SK-BR3 cell lysates using Catenin, gamma Mouse Monoclonal Antibody (15F11).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells. Catenin, gamma Mouse Monoclonal Antibody (15F11) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
