CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA
Western Blot An
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30)
Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30)
Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).
SDS-PAGE Analysis Purified Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30). Confirmation of Integrity and Purity of Antibody
Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescent staining of methanol-fixed HeLa cells. KRT7 Mouse Monoclonal Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
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