CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. MAb to cytokeratin 7 is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 mi
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/2200).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/2200).
Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/2200).
Formalin-fixed, paraffin-embedded human Placenta stained with Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/2200).
Western Blot Analysis of human HeLa cell lysate using Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/2200).
SDS-PAGE Analysis Purified Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/2200). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human SK-BR3 cell lysate using Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/2200).
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