Cytokeratin 17 (KRT17) (Basal Epithelial Marker) Antibody, IgG2b, Clone: [E3, same as Ks17.E3], Mouse, Monoclonal
Biozol Catalog Number:
NBT-3872-MSM1-P1ABX
Supplier Catalog Number:
3872-MSM1-P1ABX
Alternative Catalog Number:
NBT-3872-MSM1-P1ABX-100
Manufacturer:
NeoBiotechnologies
Host:
Mouse
Category:
Antikörper
Application:
FC, IF, IHC, WB
Species Reactivity:
Bovine, Goat, Human, Porcine, Rat
Immunogen:
Cytoskeletal fraction of rat colon epithelium
Alternative Names:
K17, Keratin Type I Cytoskeletal 17, Keratin-17, KRT17, PCHC1
Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glandssuch as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Frozen & formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with
Western blot of HeLa lysate using CK1
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with CK17 Mouse Monoclonal Antibody (E3).
Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CK17 Mouse Monoclonal Antibody (E3).
Western blot of HeLa lysate using CK17 Mouse Monoclonal Antibody (E3).
Western Blot Analysis of HeLa lysate using Cytokeratin 17 Mouse Monoclonal Antibody (E3, same as Ks17.E3)
SDS-PAGE Analysis of Purified Keratin 17 Mouse Monoclonal Antibody (E3). Confirmation of Purity and Integrity of Antibody.
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